Table 3: Analytical characteristics of the fixed time (15 min) method.
Parameters |
FVS |
λmax (nm) |
615 |
Beer's law range (µg/mL) |
3.0-36.0 |
Optimum photometric range (µg/mL) |
6.5-32.0 |
Molar absorptivity (L/mol cm) |
1.30 × 104 |
LOD (µg/mL) |
0.23 |
LOQ (µg/mL) |
0.71 |
Sandell's sensitivity (µg/cm2 per 0.001 absorbance unit) |
0.080 |
Regression equationa |
A = 0.0211C + 0.0477 |
Correlation coefficient, r |
0.9998 |
aA = mC + b, where A is the absorbance and C is the concentration in µg/mL. |