Table 1: Historical RI increment values for human plasma/serum protein.

Sample

Remarks (solvent, conditions, calibration)

dn/dc [mL/mg]

Reference

Plasma

19 plasma pools for fractionation

0.0001833

[2]

Serum

0.9% NaCl

0.0001784

[3]

Serum

15 normal sera, UF permeate-subtracted, Kjeldahl

0.000194

[4]

Serum

69 sera, gravimetric

0.000196

[5]

Serum

N/D

0.000195

[6]

Serum

20 pathological sera

0.000210

[7]

Serum

32 pathological sera

0.000197

[8]

Serum

10 pathological sera

0.000200

[9]

Serum

47 pathological sera

0.000201

[9]

Serum

39 sera, gravimetric

0.0001906

[10]

Serum

4 h after venipuncture (VP)

0.000189

[11]

Serum

Veronal buffer (VP), pH = 8.6 (dialyzed, 4 h after VP)

0.000193

[12]

Serum

VP, pH = 8.6 (dialyzed, 24 h at room temperature)

0.000204

[12]

Serum

Personal communication to authors

0.000179

[13]

Serum

Recalculated using a protein to N ratio of 6.54

0.000187

[14]

Remarks: Robertson (1912) determined a dn/dc value of 0.000195 mL/mg for bovine serum protein [15].