Table 4: Working range, Linearity, LOD, LOQ and Uncertainty of the method.
Amino Acid |
Working Range (g/100 g) |
Regression Equation |
R2 |
LOD (g/100 g) |
LOQ (g/100 g) |
% Expanded Uncertainty (k = 2) |
Asp |
0.045-3.32 |
y = 1.0164E + 06x - 3.1558E-01 |
0.999 |
0.043 |
0.045 |
4 |
Glu |
0.054-3.68 |
y = 1.3315E + 04x + 1.5022E-02 |
0.999 |
0.051 |
0.054 |
5 |
Ser |
0.035-2.62 |
y = 1.0467E + 06x - 4.5370E-01 |
0.999 |
0.034 |
0.035 |
5 |
His |
0.050-3.88 |
y = 6.0263E + 05x - 1.6916E-01 |
0.999 |
0.049 |
0.050 |
3 |
Gly |
0.025 - 1.88 |
y = 1.0718E + 06x - 4.6236E-01 |
0.999 |
0.024 |
0.025 |
4 |
Thr |
0.039 - 2.98 |
y = 1.0313E + 06x - 2.7667E-01 |
0.999 |
0.038 |
0.039 |
4 |
Arg |
0.057 - 4.35 |
y = 1.0328E + 06x - 5.3172E-01 |
0.999 |
0.056 |
0.057 |
5 |
Ala |
0.031 - 2.22 |
y = 6.6305E + 06x + 2.5812E + 00 |
0.999 |
0.030 |
0.031 |
3 |
Tyr |
0.078 - 4.52 |
y = 9.9886E + 05x - 4.4620E-01 |
0.999 |
0.069 |
0.078 |
4 |
Val |
0.046 - 2.92 |
y = 1.2085E + 06x - 2.6690E-01 |
0.999 |
0.042 |
0.046 |
7 |
Met |
0.052 - 3.72 |
y = 1.0892E + 06x - 4.5200E-01 |
0.999 |
0.050 |
0.052 |
7 |
Trp |
0.071 - 5.10 |
y = 1.9063E + 07x - 9.8928E-01 |
0.999 |
0.069 |
0.071 |
3 |
Phe |
0.056 - 4.12 |
y = 1.0562E + 06x + 1.3355E-01 |
0.999 |
0.054 |
0.056 |
3 |
Ile |
0.043 - 3.28 |
y = 1.0258E + 06x - 2.0146E-01 |
0.999 |
0.042 |
0.043 |
3 |
Leu |
0.043 - 3.28 |
y = 1.0717E + 06x - 1.7994E-01 |
0.999 |
0.042 |
0.043 |
3 |
Lys |
0.050 - 3.65 |
y = 1.5634E + 06x + 2.5580E + 00 |
0.999 |
0.048 |
0.050 |
5 |
Pro |
0.043 - 2.88 |
y = 1.2774E + 06x - 7.3935E-01 |
0.999 |
0.040 |
0.043 |
4 |
LOD: Limit of Detection; LOQ: Limit of Quantification; R2: Regression Coefficient Coverage factor (k) = 2; Asp: Aspartic acid; Glu: Glutamic acid; Ser: Serine; His: Histidine; Gly: Glycine; Thr: Threonine; Arg: Arginine; Ala: Alanine; Tyr: Tyrosine; Val: Valine; Met: Methionine; Trp: Tryptophan; Phe: Phenylalanine; Ile: Isoleucine; Leu: Leucine; Lys: Lysine; Pro: Proline