Table 4: Working range, Linearity, LOD, LOQ and Uncertainty of the method.

Amino Acid

Working Range

 (g/100 g)

Regression Equation

R2

LOD

(g/100 g)

LOQ

(g/100 g)

% Expanded

Uncertainty (k = 2)

Asp

0.045-3.32

y = 1.0164E + 06x - 3.1558E-01

0.999

0.043

0.045

4

Glu

0.054-3.68

y = 1.3315E + 04x + 1.5022E-02

0.999

0.051

0.054

5

Ser

0.035-2.62

y = 1.0467E + 06x - 4.5370E-01

0.999

0.034

0.035

5

His

0.050-3.88

y = 6.0263E + 05x - 1.6916E-01

0.999

0.049

0.050

3

Gly

0.025 - 1.88

y = 1.0718E + 06x - 4.6236E-01

0.999

0.024

0.025

4

Thr

0.039 - 2.98

y = 1.0313E + 06x - 2.7667E-01

0.999

0.038

0.039

4

Arg

0.057 - 4.35

y = 1.0328E + 06x - 5.3172E-01

0.999

0.056

0.057

5

Ala

0.031 - 2.22

y = 6.6305E + 06x + 2.5812E + 00

0.999

0.030

0.031

3

Tyr

0.078 - 4.52

y = 9.9886E + 05x - 4.4620E-01

0.999

0.069

0.078

4

Val

0.046 - 2.92

y = 1.2085E + 06x - 2.6690E-01

0.999

0.042

0.046

7

Met

0.052 - 3.72

y = 1.0892E + 06x - 4.5200E-01

0.999

0.050

0.052

7

Trp

0.071 - 5.10

y = 1.9063E + 07x - 9.8928E-01

0.999

0.069

0.071

3

Phe

0.056 - 4.12

y = 1.0562E + 06x + 1.3355E-01

0.999

0.054

0.056

3

Ile

0.043 - 3.28

y = 1.0258E + 06x - 2.0146E-01

0.999

0.042

0.043

3

Leu

0.043 - 3.28

y = 1.0717E + 06x - 1.7994E-01

0.999

0.042

0.043

3

Lys

0.050 - 3.65

y = 1.5634E + 06x + 2.5580E + 00

0.999

0.048

0.050

5

Pro

0.043 - 2.88

y = 1.2774E + 06x - 7.3935E-01

0.999

0.040

0.043

4

LOD: Limit of Detection; LOQ: Limit of Quantification; R2: Regression Coefficient Coverage factor (k) = 2; Asp: Aspartic acid; Glu: Glutamic acid; Ser: Serine; His: Histidine; Gly: Glycine; Thr: Threonine; Arg: Arginine; Ala: Alanine; Tyr: Tyrosine; Val: Valine; Met: Methionine; Trp: Tryptophan; Phe: Phenylalanine; Ile: Isoleucine; Leu: Leucine; Lys: Lysine; Pro: Proline