Table 3: Precision, recovery and selectivity.
|
Amino acid |
Precision (% RSD) (n = 6) |
% Recovery (n = 6) |
Selectivity |
||
|
Intra - day |
Inter - day |
Difference in relative RT between spiked and blank samples (min) |
Resolution (Rs) |
||
|
Asp |
2 |
3 |
94 ± 3 |
0.0010 |
17 |
|
Glu |
2 |
2 |
98 ± 2 |
0.0012 |
24 |
|
Ser |
2 |
4 |
100 ± 4 |
0.0008 |
10 |
|
His |
3 |
5 |
100 ± 5 |
0.0005 |
4 |
|
Gly |
2 |
2 |
94 ± 2 |
0.0007 |
2 |
|
Thr |
3 |
4 |
99 ± 4 |
0.0008 |
5 |
|
Arg |
3 |
4 |
90 ± 4 |
0.0004 |
9 |
|
Ala |
2 |
4 |
96 ± 4 |
0.0003 |
13 |
|
Tyr |
2 |
3 |
88 ± 3 |
0.0006 |
17 |
|
Val |
3 |
6 |
90 ± 6 |
0.0013 |
4 |
|
Met |
5 |
6 |
86 ± 6 |
0.0001 |
2 |
|
Trp |
3 |
5 |
98 ± 5 |
0.0007 |
9 |
|
Phe |
2 |
5 |
94 ± 5 |
0.0007 |
5 |
|
Ile |
2 |
3 |
95 ± 3 |
0.0005 |
2 |
|
Leu |
2 |
5 |
95 ± 5 |
0.0004 |
7 |
|
Lys |
2 |
6 |
90 ± 6 |
0.0002 |
4 |
|
Pro |
3 |
6 |
90 ± 6 |
0.0001 |
25 |
RSD: Relative Standard Deviation; RT: Retention time; n: Number of replicates; ±: Standard deviation of recoveries are given followed by the ± symbol in the respective cells; Asp: Aspartic acid; Glu: Glutamic acid; Ser: Serine; His: Histidine; Gly: Glycine; Thr: Threonine; Arg: Arginine; Ala: Alanin; Tyr: Tyrosine; Val: Valine; Met: Methionine; Trp: Tryptophan; Phe: Phenylalanine; Ile: Isoleucine; Leu: Leucine; Lys: Lysine; Pro: Proline