Line

Function

Amount

Vial position

Reagent

1

Draw

2.5 μL

1

borate buffer

2

Draw

0.5μL

7 onwards^*

sample

3

Mix

3.0 in air

max speed

2 times

4

Wait

0.5 min

5

Draw

0 μL

2

Water - (needle wash using uncapped vial)

6

Draw

0.5 μL

3

OPA

7

Mix

3.5 μL in air

Max speed

6 times

8

Draw

0.5 μL

4

FMOC

9

Mix

4.0 μL in air

Max speed

6 times

10

Draw

0 μL

2

Water- (needle wash using water in uncapped vial)

11

Draw

6

acetonitrile

12

Draw

32 μL

5

water (capped vial)

13

Mix

18 μL in air

max speed

2 times

14

Inject